Generate Quiz 68E81DCF0AAB9

Given that it contains every tool needed to engineer a plant cell, this choice is accurate. To remove a fragment of DNA, restriction enzymes are employed. The cut-out gene is engineered and transferred into the protoplast using DNA ligases.

A vector in molecular cloning is a DNA molecule that is used to artificially transfer foreign genetic material into a different cell so that it can be expressed and/or replicated. Recombinant DNA is a vector that contains foreign DNA. Since the vector should be small in order to facilitate easy integration into the host cell, it is appropriate to incorporate it with the large external DNA fragment. It ought to be able to insert a sizable DNA segment. It ought to have several cloning sites.

Through the repair or reconstruction of damaged genetic material, gene therapy is a branch of medicine that focuses on genetically altering cells to produce a therapeutic effect or to treat disease. Cancer, scid, and cystic fibrosis can all be treated with gene therapy. However, it doesn’t cure tuberculosis.

Adding a particular DNA sequence to a plant’s genome to give it new or different traits is known as genetic modification. This can entail altering the plant’s growth pattern or conferring disease resistance.

By permitting the DNA to replicate with host DNA, the number of recombinant DNA copies can be increased.

Because modified Lambda phages are effective at packing and injecting DNA into cells, they are used as a vector to introduce foreign DNA into cells.

In a criminal investigation, a laboratory technique called DNA fingerprinting is used to connect biological evidence to a suspect. A suspect’s DNA sample and a sample collected from a crime scene are compared and examined. The evidence originated from that suspect if the two DNA profiles match.

The right response is this choice. Liposomes, which are microscopic vesicles, can be used as a delivery system to introduce a healthy gene into diseased cells in cystic fibrosis. The goal of this method, called gene therapy, is to substitute a functional CFTR gene for the defective one that causes cystic fibrosis.

The artificial process of growing plants vegetatively using tissue culture or cell culture methods is known as micropropagation. Asexual reproduction or vegetative propagation are the two methods used to create plants in vitro in this artificial propagation process.

The absence of Cl-protein channels causes a reduction in the flow of chloride ions and water out of cells, resulting in thicker mucus in cystic fibrosis.

DNA recombinant technology depends on enzymes, which aid in the necessary modification and alteration of DNA molecules.

CaCl2: This is the right response. A procedure known as “calcium chloride transformation” involves treating bacterial cells with a calcium chloride (CaCl2) solution to improve their capacity to absorb recombinant plasmids. By temporarily perforating the bacterial cell membrane, the CaCl2 treatment facilitates the plasmids’ entry into the cells.

Certain genes can be separated using restriction enzymes, which are used to separate DNA molecules at particular locations.

Genetic engineering is the process of changing the genetic material by either adding or deleting elements.

Reverse transcriptase’s general function is to change RNA sequences into cDNA sequences that can be inserted into various genomic locations.

One lab technique for sorting DNA, RNA, or protein mixtures based on molecular size is gel electrophoresis. An electrical field forces the molecules to be separated through a gel with tiny pores in gel electrophoresis.

recall of facts;

The antibiotic resistance genes ‘bla’, which codes for the ampicillin resistance (AmpR) protein, and ‘tetA’, which codes for the tetracycline resistance (TetR) protein, are found in pBR322, which is base pairs long.

One method for separating DNA fragments based on size and speed is gel electrophoresis.An electric current is used to draw DNA samples through a gel after they have been loaded into wells (indentations) at one end of the gel. Due to their negative charge, DNA fragments gravitate toward the positive electrode.

DNA from two or more sources is combined to create recombinant DNA, or rDNA.

DNA probes are single-stranded DNA segments that are used to hybridize and find complementary nucleic acid sequences, or target sequences.

An enzyme that cleaves DNA into fragments at or close to particular recognition sites within molecules known as restriction sites is called a restriction enzyme, restriction endonuclease, or restrictase.

Any alteration to a DNA sequence is called a mutation. Errors in DNA copying that occur during cell division, ionizing radiation exposure, exposure to chemicals known as mutagens, or viral infection can all cause mutations.

The right response is that the nucleotide base pairs are similar. Uracil is an RNA nucleotide that has nothing to do with DNA.Deoxyribose sugar has the same structure everywhere.