Generate Quiz 68E81DD36723E

DNA fingerprinting uses genetic marker probes to find markers like microsatellites, which result in unique patterns on X-ray and autoradiography films.

It is employed to create millions of copies of a single DNA molecule or a small number of them. The target DNA sequence is extremely specific; it may make up less than one part in a million of the entire DNA sample.

In a criminal investigation, a laboratory technique called DNA fingerprinting is used to connect biological evidence to a suspect. A suspect’s DNA sample and a sample collected from a crime scene are compared. Paternity can also be established through DNA fingerprinting. A DNA fingerprint is depicted in the figure below:

Through the repair or reconstruction of damaged genetic material, gene therapy is a branch of medicine that focuses on genetically altering cells to produce a therapeutic effect or to treat disease. Cancer, scid, and cystic fibrosis can all be treated with gene therapy. However, it doesn’t cure tuberculosis.

Liposome-based gene therapy is used to treat cystic fibrosis. Liposomes are tiny lipoprotein vesicles that are used to transfer the healthy gene into the patient’s cells.

Plasmids have been exploited by scientists as instruments for gene manipulation, transfer, and cloning. Thus, each of these is accurate.

Gene transfer between organisms is now feasible thanks to recombinant DNA (rDNA) technology.

A laboratory technique called DNA fingerprinting uses the nucleotide sequences of specific, individual-specific regions of human DNA to infer a person’s likely identity. Gene therapy is unrelated to DNA fingerprinting.

The right response is this choice. Liposomes, which are microscopic vesicles, can be used as a delivery system to introduce a healthy gene into diseased cells in cystic fibrosis. The goal of this method, called gene therapy, is to substitute a functional CFTR gene for the defective one that causes cystic fibrosis.

Adenosine Deaminase (ADA) deficiency causes “SCID,” so it is used to treat the condition. Option A is therefore the right choice. Digestion is aided by pancreatic enzymes (B). In some organisms, beta-galactosidase (C) converts lactose to galactose and glucose. Bacteria produce an enzyme called beta-lactamase (D), which breaks down the beta-lactam ring found in beta-lactam medications like penicillin, giving them resistance to these medications.

One method for separating DNA fragments based on size and speed is gel electrophoresis.An electric current is used to draw DNA samples through a gel after they have been loaded into wells (indentations) at one end of the gel. Due to their negative charge, DNA fragments gravitate toward the positive electrode.

Cloning is an asexual reproduction technique that does not involve inter-gamete contact or fertilization.

PCR does not require the presence of ribonucleotides. Instead of using ribonucleotides, PCR uses deoxyribonucleotide triphosphates (dATP, dCTP, dGTP, and dTTP) to synthesize DNA.

DNA bands can be found using fluorescent dyes, sometimes referred to as reactive dyes or fluorophores. By using the molecule’s inherent fluorescence or by attaching it with a fluorophore, fluorescence is used in biology as a non-destructive method of analyzing biological molecules, even at low concentrations. As a result, DNA bands are easily visible.

The right response is that the nucleotide base pairs are similar. Uracil is an RNA nucleotide that has nothing to do with DNA.Deoxyribose sugar has the same structure everywhere.

This is the right response. In order to produce a distinct “fingerprint” or pattern, DNA fingerprinting entails the molecular examination of particular DNA regions. It is frequently employed for forensic identification, paternity testing, and other purposes.

The first step involves extracting the mRNA, which is then reversed to create a copy of the DNA. Step three involves cutting DNA with a restriction enzyme, step four involves joining donor DNA to plasmid RNA with a ligase enzyme, and step five involves inserting the recombinant plasmid into the cell.

In order to test the safety of vaccines before they are administered to humans, transgenic mice are being created. The safety of the polio vaccine is being tested in transgenic mice. In order to better understand how genes contribute to the development of disease and to investigate potential new treatments, many transgenic animals are created. Many human diseases, including cancer, rheumatoid arthritis, Alzheimer’s disease, hemophilia, thalassemia, and cystic fibrosis, now have transgenic models available.

Combining DNA molecules from two distinct species is known as recombinant DNA technology. In order to create novel genetic combinations useful for industry, agriculture, science, and medicine, the recombinant DNA molecule is introduced into a host organism.